1Y4M
Crystal structure of human endogenous retrovirus HERV-FRD envelope protein (syncitin-2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.95375 |
| Spacegroup name | I 4 |
| Unit cell lengths | 90.404, 90.404, 38.654 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.960 - 1.600 |
| R-factor | 0.248 |
| Rwork | 0.248 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mof |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.580 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Number of reflections | 22623 | |
| Completeness [%] | 94.9 | 75.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 292 | Sodium Chloride, Sodium Acetate tri-hydrate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
