1Y4J
Crystal structure of the paralogue of the human formylglycine generating enzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-04-26 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.078, 100.750, 62.704 |
| Unit cell angles | 90.00, 101.68, 90.00 |
Refinement procedure
| Resolution | 32.000 - 1.864 |
| R-factor | 0.17185 |
| Rwork | 0.170 |
| R-free | 0.20548 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.338 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Number of reflections | 47438 | |
| <I/σ(I)> | 22.7 | 1.8 |
| Completeness [%] | 98.7 | 87.3 |
| Redundancy | 3.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | Tris/HCl, MPD, CaCl2, PEG8000, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
