1Y43
crystal structure of aspergilloglutamic peptidase from Aspergillus niger
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 283 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-11-30 |
| Detector | FUJI |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.100, 70.700, 38.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.400 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.22600 |
| Structure solution method | MIR |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | MLPHARE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.440 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 29424 | |
| Completeness [%] | 97.5 | 82.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 2.1 | 298 | ammonium sulfate, dimethyl sulfoxide, glycine, pH 2.1, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
