1Y2T
Crystal structure of the common edible mushroom (Agaricus bisporus) lectin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-07-24 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 91.935, 96.681, 75.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.940 - 1.500 |
| R-factor | 0.18887 |
| Rwork | 0.187 |
| R-free | 0.20298 |
| Structure solution method | MIR |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.903 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.500 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 53757 | |
| <I/σ(I)> | 7.9 | 7.1 |
| Completeness [%] | 99.5 | 97.6 |
| Redundancy | 6.4 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | Sodium formate, Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
