1Y2M

Crystal structure of phenylalanine ammonia-lyase from yeast Rhododporidium toruloides

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-04-22
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9794
Spacegroup name	P 21 21 21
Unit cell lengths	104.759, 151.612, 179.922
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.880 - 1.600
R-factor	0.15933
Rwork	0.158
R-free	0.18653
Structure solution method	SAD
RMSD bond length	0.016
RMSD bond angle	1.556
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHELXS
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.500
High resolution limit [Å]	1.470	1.470
Number of reflections	723300
Completeness [%]	99.2	80.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	macroseeding	6.5	277	mPEG5000, Tris, pH 6.5, macroseeding, temperature 277K