1Y1M
Crystal structure of the NR1 ligand binding core in complex with cycloleucine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2003-10-31 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.156, 53.203, 67.546 |
| Unit cell angles | 110.12, 91.55, 108.69 |
Refinement procedure
| Resolution | 15.000 - 1.800 |
| R-factor | 0.191 |
| Rwork | 0.187 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.185 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.770 | 1.860 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.059 | 0.250 |
| Number of reflections | 59649 | |
| <I/σ(I)> | 3.8 | 3.69 |
| Completeness [%] | 95.2 | 95.2 |
| Redundancy | 3.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | PEG, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
