1Y12
Structure of a hemolysin-coregulated protein from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 105 |
| Wavelength(s) | 0.97943, 0.97956, 0.96863 |
| Spacegroup name | P 6 |
| Unit cell lengths | 146.729, 146.729, 42.222 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.950 |
| R-factor | 0.18174 |
| Rwork | 0.179 |
| R-free | 0.22665 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.150 |
| Data reduction software | d*TREK |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.030 | 2.040 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 37749 | |
| Completeness [%] | 98.4 | 91.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | PEG 3350, HEPES, tri-sodium citrate, Peritone N, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
