1Y10
Mycobacterial adenylyl cyclase Rv1264, holoenzyme, inhibited state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-09-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9393 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.936, 146.830, 84.849 |
| Unit cell angles | 90.00, 105.26, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.300 |
| R-factor | 0.19287 |
| Rwork | 0.190 |
| R-free | 0.24230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Rv1264 RESIDUES 1-207 (to be deposited) |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.222 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.330 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.092 | 0.293 |
| Number of reflections | 63500 | |
| <I/σ(I)> | 12.9 | 1.8 |
| Completeness [%] | 98.0 | 84.1 |
| Redundancy | 6.1 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | PEG400, calcium acetate, sodium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP |
