1Y0Q
Crystal structure of an active group I ribozyme-product complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 8-BM |
| Synchrotron site | APS |
| Beamline | 8-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-08-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 94.570, 140.970, 210.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.540 - 3.600 |
| R-factor | 0.277 |
| Rwork | 0.277 |
| R-free | 0.31000 |
| Structure solution method | MIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | DM |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.730 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Number of reflections | 15937 | |
| <I/σ(I)> | 3.8 | |
| Completeness [%] | 98.0 | 99.7 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | potassium cacodylate, magnesium, spermine, lithium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | potassium cacodylate | ||
| 2 | 1 | 1 | magnesium | ||
| 3 | 1 | 1 | spermine | ||
| 4 | 1 | 1 | lithium sulfate |
