1Y0K
Structure of Protein of Unknown Function PA4535 from Pseudomonas aeruginosa strain PAO1, Monooxygenase Superfamily
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2004-09-30 |
| Detector | SBC-2 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.422, 53.918, 55.583 |
| Unit cell angles | 90.00, 119.41, 90.00 |
Refinement procedure
| Resolution | 48.390 - 1.750 |
| R-factor | 0.188 |
| Rwork | 0.186 |
| R-free | 0.22200 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.167 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.800 |
| High resolution limit [Å] | 1.750 | 1.740 |
| Rmerge | 0.050 | |
| Number of reflections | 18416 | |
| Completeness [%] | 98.2 | 86.5 |
| Redundancy | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 298 | 1.5M Na Acetate, 0.1M Na Cacodylate, ph 6.5, VAPOR DIFFUSION, temperature 298K, pH 6.50 |
