1Y0E
Crystal structure of putative ManNAc-6-P epimerase from Staphylococcus aureus (strain N315)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-19 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97951 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.961, 76.283, 183.661 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 91.670 - 1.950 |
| R-factor | 0.17933 |
| Rwork | 0.177 |
| R-free | 0.21564 |
| Structure solution method | SAD |
| RMSD bond length | 0.037 |
| RMSD bond angle | 2.337 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.142 | 0.539 |
| Number of reflections | 54814 | |
| <I/σ(I)> | 29.7 | 3.21 |
| Completeness [%] | 98.7 | 87.5 |
| Redundancy | 19.1 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | NaH2PO4, K2HPO4, imidazole, NaCl, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
