1XZO
Identification of a disulfide switch in BsSco, a member of the Sco family of cytochrome c oxidase assembly proteins
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12B |
Synchrotron site | NSLS |
Beamline | X12B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-06-27 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97960 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 68.033, 68.033, 191.733 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.200 - 1.702 |
R-factor | 0.24088 |
Rwork | 0.238 |
R-free | 0.28935 |
Structure solution method | SAD |
RMSD bond length | 0.031 |
RMSD bond angle | 2.388 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.550 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 54348 | |
Completeness [%] | 0.94 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG5K MME, CdCl2, CaCl2, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |