1XYZ
A COMMON PROTEIN FOLD AND SIMILAR ACTIVE SITE IN TWO DISTINCT FAMILIES OF BETA-GLYCANASES
Experimental procedure
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X31 |
Detector technology | IMAGE PLATE |
Collection date | 1993-11 |
Detector | MARRESEARCH |
Wavelength(s) | 0.92 |
Spacegroup name | P 1 |
Unit cell lengths | 47.100, 51.100, 70.740 |
Unit cell angles | 100.54, 83.79, 101.64 |
Refinement procedure
Resolution | 10.000 - 1.400 |
R-factor | 0.183 |
Rwork | 0.183 |
R-free | 0.22100 |
RMSD bond length | 0.015 |
RMSD bond angle | 23.200 * |
Data reduction software | DENZO |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 10.000 * |
High resolution limit [Å] | 1.400 * |
Rmerge | 0.083 |
Total number of observations | 343930 * |
Number of reflections | 117046 * |
Completeness [%] | 96.4 |
Redundancy | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.4 * | 24 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 20 (mM) | |
3 | 1 | reservoir | PEG2000 | 20 (%) | |
4 | 1 | reservoir | calcium acetate | 0.2 (M) | |
5 | 1 | reservoir | Tris-HCl | 0.1 (M) |