1XYH
Crystal Structure of Recombinant Human Cyclophilin J
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2004-06-26 |
| Detector | BRUKER |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 41.309, 41.309, 172.116 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.510 - 2.600 |
| R-factor | 0.175 |
| Rwork | 0.175 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cpl |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.510 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.084 | 0.372 |
| Number of reflections | 5560 | |
| Completeness [%] | 95.9 | 92.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 293 | Tris-HCl, PEG8000, DMSO, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
