1XY3
Urate oxidase from aspergillus flavus complexed with guanine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS |
| Temperature [K] | 291 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.753, 141.936, 135.082 |
| Unit cell angles | 90.00, 92.67, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.200 |
| Rwork | 0.172 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wwr |
| RMSD bond length | 0.007 |
| RMSD bond angle | 18.828 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.300 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.330 | |
| Number of reflections | 48327 | |
| <I/σ(I)> | 9.4 | 5.6 |
| Completeness [%] | 93.9 | 96 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 8.5MG/ML PROTEIN, 0.2MG/ML GUANINE, 5-7%(W/V) PEG8000, 100MM TRIS/HCL, pH 8.00, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
