1XXQ
Structure of a mannose-specific jacalin-related lectin from Morus nigra
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-04-16 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9116 |
Spacegroup name | P 65 |
Unit cell lengths | 110.740, 110.740, 159.280 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 21.850 - 1.800 |
R-factor | 0.19 |
Rwork | 0.190 |
R-free | 0.21000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.042 | 0.160 |
Number of reflections | 96741 | |
<I/σ(I)> | 20.84 | 4.96 |
Completeness [%] | 90.6 | 70.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | ammonium sulphate, imidazole buffer, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |