1XXJ

Urate oxidase from aspergillus flavus complexed with 5-amino 6-nitro uracil

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LURE BEAMLINE DW32
Synchrotron site	LURE
Beamline	DW32
Temperature [K]	291
Detector technology	IMAGE PLATE
Collection date	2003-11-30
Detector	MARRESEARCH
Wavelength(s)	0.972
Spacegroup name	P 21 21 2
Unit cell lengths	126.245, 142.265, 81.322
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.800
Rwork	0.189
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wrr
RMSD bond length	0.012
RMSD bond angle	1.108
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	BUSTER-TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.880
High resolution limit [Å]	2.800	2.800
Number of reflections	36394
<I/σ(I)>	16.8	10.1
Completeness [%]	98.7	98.4
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	8.5MG/ML PROTEIN, 0.2MG/ML DIAMINOURACIL, REM 5-7%(W/V) PEG 8000, 100MM TRIS/HCL, PH 8.0, NACACODYLATE 100mM pH 7.0, 1mM DTT, 1mM cymelarsan, pH 8.00, VAPOR DIFFUSION, SITTING DROP, temperature 291K