1XXG

Crystal Structure of Staphylococcal Enterotoxin G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-06-10
Detector	ADSC QUANTAM Q315
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	70.738, 70.738, 111.898
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.700 - 2.200
R-factor	0.231
Rwork	0.231
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3sbb
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.700	2.280
High resolution limit [Å]	2.200	2.200
Number of reflections	15015
Completeness [%]	99.9	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	PEG 4000, ammonium sulphate, sodium acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K