1XWW
Crystal Structure of Human B-form Low Molecular Weight Phosphotyrosyl Phosphatase at 1.6 Angstrom Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-18 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.258, 35.481, 60.375 |
| Unit cell angles | 90.00, 99.97, 90.00 |
Refinement procedure
| Resolution | 59.760 - 1.630 |
| R-factor | 0.16371 |
| Rwork | 0.158 |
| R-free | 0.21566 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5pnt |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.762 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.800 | 1.680 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Number of reflections | 15805 | |
| Completeness [%] | 95.9 | 88.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEGmme 5000, ammonium sulfate, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
