1XWR

Crystal structure of the coliphage lambda transcription activator protein CII

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Wavelength(s)	0.9836, 0.9841, 0.9949, 0.9733
Spacegroup name	C 2 2 21
Unit cell lengths	63.907, 106.794, 119.784
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.560
R-factor	0.25
Rwork	0.250
R-free	0.28600
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.590
High resolution limit [Å]	2.550	2.550
Rmerge	0.068	0.710
Number of reflections	13650
<I/σ(I)>	22.4	2.1
Completeness [%]	99.8	99.4
Redundancy	6.5	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.2	298	PEG 3350, isopropanol, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
1	VAPOR DIFFUSION, HANGING DROP	8.2	298	PEG 3350, isopropanol, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
1	VAPOR DIFFUSION, HANGING DROP	8.2	298	PEG 3350, isopropanol, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K