1XWM
The crystal structure of PhoU (phosphate uptake regulator), Structural genomics
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-19 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9795, 0.94656 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 61.172, 61.172, 121.264 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.590 - 2.500 |
| R-factor | 0.243 |
| Rwork | 0.243 |
| R-free | 0.33400 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.300 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.104 | 0.667 |
| Number of reflections | 15295 | |
| <I/σ(I)> | 22.6 | 1.87 |
| Completeness [%] | 99.7 | 98.6 |
| Redundancy | 8.5 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 298 | 16% PEG3350, 0.2M ammonium tartrate, 0.1M Bis-Tris buffer, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
