1XWJ

Vinculin head (1-258) in complex with the talin vinculin binding site 3 (1945-1969)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2004-02-23
Detector	ADSC QUANTUM 4
Wavelength(s)	0.979625, 0.972447, 0.979404
Spacegroup name	P 21 21 2
Unit cell lengths	52.173, 72.434, 96.372
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.950 - 2.600
Rwork	0.241
R-free	0.28100
Structure solution method	MAD
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.099	0.211
Number of reflections	11765
Completeness [%]	92.2	78.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	1M NaH2PO4, 1M K2HPO4, 0.1M CAPS, pH 10.9, 3% Hexanediol, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K