1XV2
Crystal Structure of a Protein of Unknown Function Similar to Alpha-acetolactate Decarboxylase from Staphylococcus aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-15 |
| Detector | SBC-2 |
| Wavelength(s) | 0.979562 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.434, 160.005, 68.056 |
| Unit cell angles | 90.00, 107.55, 90.00 |
Refinement procedure
| Resolution | 30.240 - 2.000 |
| R-factor | 0.191 |
| Rwork | 0.191 |
| R-free | 0.23600 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Phasing software | HKL-3000 |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.067 | |
| Number of reflections | 126804 | |
| Completeness [%] | 93.7 | |
| Redundancy | 2 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 0.20M lithium sulphate, 0.10M tris pH 8.5, 25% polyethylene glycol 3350, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
