1XU9
Crystal Structure of the Interface Closed Conformation of 11b-hydroxysteroid dehydrogenase isozyme 1
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-06-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.428, 152.673, 73.918 |
Unit cell angles | 90.00, 93.77, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.550 |
R-factor | 0.15801 |
Rwork | 0.157 |
R-free | 0.18085 |
Structure solution method | MAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.558 |
Data reduction software | HKL-2000 |
Data scaling software | CCP4 ((TRUNCATE)) |
Phasing software | SHARP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.610 |
High resolution limit [Å] | 1.550 | 1.550 |
Number of reflections | 169082 | |
<I/σ(I)> | 20.9 | 2.2 |
Completeness [%] | 88.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 298 | PEG, MES buffer, pH 7.0, VAPOR DIFFUSION, temperature 298K |