1XTK
structure of DECD to DEAD mutation of human UAP56
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-16 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.432, 49.756, 62.805 |
| Unit cell angles | 95.57, 101.93, 110.89 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| R-factor | 0.25 |
| Rwork | 0.250 |
| R-free | 0.30000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 1XTI |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.298 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 14805 | |
| Completeness [%] | 99.0 | 92.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 290 | iso-propanol, tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K, pH 7.50 |
