1XSE
Crystal Structure of Guinea Pig 11beta-Hydroxysteroid Dehydrogenase Type 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-09-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.092 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 118.385, 118.385, 184.772 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.500 |
| R-factor | 0.19608 |
| Rwork | 0.192 |
| R-free | 0.26729 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.761 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0001) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 200.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 22866 | |
| <I/σ(I)> | 15.5 | 3.7 |
| Completeness [%] | 99.5 | 97.8 |
| Redundancy | 5.6 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 291 | PEG 550 MME, BisTris, pH 6.5, vapor diffusion, temperature 291K |
| 1 | VAPOR DIFFUSION | 6.5 | 291 | PEG 550 MME, BisTris, pH 6.5, vapor diffusion, temperature 291K |
