1XQF
The mechanism of ammonia transport based on the crystal structure of AmtB of E. coli.
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-31 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9797 |
| Spacegroup name | P 63 |
| Unit cell lengths | 111.800, 111.800, 86.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 1.800 |
| R-factor | 0.18196 |
| Rwork | 0.181 |
| R-free | 0.20755 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.121 |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.670 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 54526 | |
| <I/σ(I)> | 13.2 | 2 |
| Completeness [%] | 95.6 | 98.5 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | PEG 550, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
