1XQE
The mechanism of ammonia transport based on the crystal structure of AmtB of E. coli.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9797 |
| Spacegroup name | H 3 |
| Unit cell lengths | 116.632, 116.632, 130.543 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 60.000 - 2.100 |
| R-factor | 0.16051 |
| Rwork | 0.159 |
| R-free | 0.18603 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | The same structure in the absence of ammonium |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.236 |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.060 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 38583 | |
| <I/σ(I)> | 13.6 | 2.5 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 5.8 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | PEG 550, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
