1XQ7
Cyclophilin from Trypanosoma cruzi bound to cyclosporin A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-22 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 |
| Unit cell lengths | 52.269, 57.059, 58.807 |
| Unit cell angles | 110.49, 108.21, 105.40 |
Refinement procedure
| Resolution | 49.390 - 2.070 |
| R-factor | 0.172 |
| Rwork | 0.169 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xo7 CYCLOPHILIN |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.056 |
| Data reduction software | ELVES |
| Data scaling software | ELVES |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.120 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Number of reflections | 29952 | |
| Completeness [%] | 94.4 | 94.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 50MM POTASSIUM PHOSPHATE, 40% PEG 8000, 2MM CYCLOSPORIN A, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 25K, TEMPERATURE 298K |
