1XPH
Structure of DC-SIGNR and a portion of repeat domain 8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-11-28 |
Detector | CUSTOM-MADE |
Wavelength(s) | 1.0395 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.233, 54.887, 62.352 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.400 - 1.410 |
R-factor | 0.17698 |
Rwork | 0.176 |
R-free | 0.19295 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1k9j |
RMSD bond length | 0.015 |
RMSD bond angle | 1.366 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.400 | 1.480 |
High resolution limit [Å] | 1.410 | 1.410 |
Number of reflections | 25340 | |
<I/σ(I)> | 5.6 | |
Completeness [%] | 97.5 | 99.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 100mM MgCl2, 100mM Na Cacodylate, 12% PEG 3000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |