1XPB

STRUCTURE OF BETA-LACTAMASE TEM1

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Detector technology	AREA DETECTOR
Detector	SIEMENS
Spacegroup name	P 21 21 21
Unit cell lengths	41.786, 62.721, 88.841
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	5.000 - 1.900
R-factor	0.156
Rwork	0.156
R-free	0.15800
RMSD bond length	0.013
RMSD bond angle	24.200 *
Data reduction software	XENGEN
Data scaling software	XENGEN
Phasing software	X-PLOR (3.87)
Refinement software	X-PLOR (3.87)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	3.340 *
High resolution limit [Å]	1.840 *	1.840 *
Rmerge	0.042 *
Number of reflections	17252	1610 *
Completeness [%]	82.2 *	47.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *	292 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	imidazole	0.1 (M)
3	1	reservoir		10 (mM)
4	1	reservoir	ammonium sulfate	43-48 (%sat)