1XP7
HIV-1 subtype F genomic RNA Dimerization Initiation Site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 90 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 27.380, 116.190, 94.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.500 |
R-factor | 0.219 |
Rwork | 0.219 |
R-free | 0.23000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.900 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.500 |
Number of reflections | 5206 |
<I/σ(I)> | 33 |
Completeness [%] | 99.6 |
Redundancy | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 310 | PEG8000, Ammonium sulfate, MgCl2, Na Cacodylate, spermine, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 310K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG8000 | ||
10 | 1 | 2 | spermine | ||
2 | 1 | 1 | Ammonium sulfate | ||
3 | 1 | 1 | MgCl2 | ||
4 | 1 | 1 | Na Cacodylate | ||
5 | 1 | 1 | spermine | ||
6 | 1 | 2 | PEG8000 | ||
7 | 1 | 2 | Ammonium sulfate | ||
8 | 1 | 2 | MgCl2 | ||
9 | 1 | 2 | Na Cacodylate |