1XP0
Catalytic Domain Of Human Phosphodiesterase 5A In Complex With Vardenafil
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2003-09-04 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 89.662, 89.662, 222.002 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 76.700 - 1.790 |
| R-factor | 0.194 |
| Rwork | 0.193 |
| R-free | 0.20788 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.369 |
| Data scaling software | ELVES |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.1.25) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.700 | 1.836 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.058 | 0.599 |
| Number of reflections | 46654 | |
| <I/σ(I)> | 7.8 | 1.3 |
| Completeness [%] | 96.9 | 96.9 |
| Redundancy | 6 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | Sodium formate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
