1XOD

Crystal structure of X. tropicalis Spred1 EVH-1 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.2
Synchrotron site	SRS
Beamline	PX14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-05-22
Detector	ADSC QUANTUM 4
Wavelength(s)	0.975
Spacegroup name	P 1 21 1
Unit cell lengths	32.811, 38.193, 79.907
Unit cell angles	90.00, 95.68, 90.00

Refinement procedure

Resolution	79.060 - 1.150
R-factor	0.15538
Rwork	0.154
R-free	0.17700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdb entries 1EVH 1i7a 1qc6
RMSD bond length	0.009
RMSD bond angle	1.335
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.000	1.170
High resolution limit [Å]	1.150	1.150
Number of reflections	68977
<I/σ(I)>	19.4	3.99
Completeness [%]	97.7	94.5
Redundancy	3.9	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	PEG 3350, potassium fluoride, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K