1XNW
Acyl-CoA Carboxylase Beta Subunit from S. coelicolor (PccB), apo form #2, mutant D422I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-09 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.850, 219.492, 135.691 |
| Unit cell angles | 90.00, 102.99, 90.00 |
Refinement procedure
| Resolution | 49.050 - 2.600 |
| R-factor | 0.2611 |
| Rwork | 0.261 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | homology model of PccB |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.050 | 2.650 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 139417 | |
| <I/σ(I)> | 8.5 | 1.4 |
| Completeness [%] | 100.0 | 99.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 35% MPD, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
