1XMH

Structure of Co(II) reconstituted methane monooxygenase hydroxylase from M. capsulatus (Bath)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-03-01
Detector	MARRESEARCH
Wavelength(s)	1.080
Spacegroup name	P 21 21 21
Unit cell lengths	71.444, 171.842, 220.936
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.550 - 2.320
R-factor	0.1941
Rwork	0.194
R-free	0.22500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB 1FZ1
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.240
High resolution limit [Å]	2.100	2.100
Rmerge	0.057	0.388
Number of reflections	159323
<I/σ(I)>	12.2	2.9
Completeness [%]	97.7	90.4
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	278	MOPS, PEG 8000, calcium chloride, sodium azide, glycerol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 278K