1XM5

Crystal structure of metal-dependent hydrolase ybeY from E. coli, Pfam UPF0054

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	110
Detector technology	CCD
Collection date	2004-06-28
Detector	MARRESEARCH
Wavelength(s)	0.97919
Spacegroup name	P 21 21 21
Unit cell lengths	46.073, 119.613, 132.903
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 2.700
R-factor	0.239
Rwork	0.234
R-free	0.27300
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	20911
Completeness [%]	97.9	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	278	PEG3350, magnesium chloride, Bis Tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 278K
1	VAPOR DIFFUSION, HANGING DROP	6.5	278	PEG3350, magnesium chloride, Bis Tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 278K