1XL6

Intermediate gating structure 2 of the inwardly rectifying K+ channel KirBac3.1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Spacegroup name	P 21 21 2
Unit cell lengths	105.756, 107.532, 89.654
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.850
Rwork	0.270
R-free	0.31000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1p7b
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.700
Number of reflections	28510

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6	293	90mM Hepes, 2.5% PEG 8000, 2.5% PEG 4000, 20% PEG 400, 12.5mM magnesium chloride, 42.5mM magnesium acetate, 2.5% glycerol, 14mM Hega-10 , pH 7.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K