1XKS
The crystal structure of the N-terminal domain of Nup133 reveals a beta-propeller fold common to several nucleoporins
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-01-28 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 84.588, 84.588, 139.048 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.460 - 2.350 |
R-factor | 0.2 |
Rwork | 0.196 |
R-free | 0.23600 |
Structure solution method | SAD |
Starting model (for MR) | Model built by SAD data from SeMet derivative |
RMSD bond length | 0.025 |
RMSD bond angle | 2.167 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.500 | 2.390 |
High resolution limit [Å] | 2.350 | 2.350 |
Number of reflections | 20899 | |
Completeness [%] | 96.5 | 93.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.7 | 277 | PEG 3350, lithium sulfate, bis-tris, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K, pH 5.70 |