1XKH
Pyoverdine outer membrane receptor FpvA from Pseudomonas aeruginosa PAO1 bound to pyoverdine
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-10-03 |
Detector | MARRESEARCH |
Wavelength(s) | 0.979413, 0.979632, 0.977801 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 137.451, 231.342, 121.695 |
Unit cell angles | 90.00, 104.57, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.600 |
Rwork | 0.259 |
R-free | 0.28800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Ferrichrome outer membrane receptor FhuA from Escherichia coli. |
RMSD bond length | 0.010 |
RMSD bond angle | 1.559 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.700 |
High resolution limit [Å] | 3.600 | 3.600 |
Number of reflections | 41896 | |
<I/σ(I)> | 8.46 | 1.14 |
Completeness [%] | 98.4 | 96 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 295 | 13-16% PEG 4000, 20-25% ethylene glycol as cryoprectant in a 0.1M sodium citrate buffer, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 295K |