1XJU
Crystal structure of secreted inactive form of P1 phage endolysin Lyz
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.349, 59.786, 76.228 |
| Unit cell angles | 90.00, 102.51, 90.00 |
Refinement procedure
| Resolution | 46.620 - 1.070 |
| R-factor | 0.134 |
| Rwork | 0.133 |
| R-free | 0.15300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.917 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.110 |
| High resolution limit [Å] | 1.070 | 2.310 | 1.070 |
| Rmerge | 0.057 | 0.032 | 0.318 |
| Number of reflections | 116001 | ||
| Completeness [%] | 90.2 | 98.7 | 51.9 |
| Redundancy | 5.6 | 5.3 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.5 | 291.15 | sodium acetate, PEG4000, pH 4.5, VAPOR DIFFUSION, temperature 291.15K |
