1XJT
Crystal structure of active form of P1 phage endolysin Lyz
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Detector technology | CCD |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.979316, 0.97945, 0.953 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 66.871, 66.871, 166.796 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.750 |
| R-factor | 0.209 |
| Rwork | 0.208 |
| R-free | 0.23800 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.485 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 3.770 | 1.750 |
| Rmerge | 0.064 | 0.051 | 0.406 |
| Number of reflections | 22274 | ||
| Completeness [%] | 96.1 | 96.5 | 91 |
| Redundancy | 17.4 | 18.6 | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.5 | 291.15 | PEG400, Sodium citrate, pH 4.5, VAPOR DIFFUSION, temperature 291.15K |
| 1 | VAPOR DIFFUSION | 4.5 | 291.15 | PEG400, Sodium citrate, pH 4.5, VAPOR DIFFUSION, temperature 291.15K |
