1XIU
Crystal structure of the agonist-bound ligand-binding domain of Biomphalaria glabrata RXR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-05-21 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 87.100, 87.100, 320.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.400 - 2.500 |
| R-factor | 0.1958 |
| Rwork | 0.193 |
| R-free | 0.24332 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fby |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.589 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.650 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 25831 | |
| <I/σ(I)> | 19.5 | 5.98 |
| Completeness [%] | 99.1 | |
| Redundancy | 15.6 | 15.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | HEPES, sodium formate, sodium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
