1XIO
Anabaena sensory rhodopsin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-01-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8984 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 103.095, 116.513, 56.186 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| Rwork | 0.230 |
| R-free | 0.25590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jgj |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.051 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.097 | 0.511 |
| Number of reflections | 23141 | |
| <I/σ(I)> | 18 | 2.4 |
| Completeness [%] | 99.0 | 93.8 |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | cubic lipid phase | 5.6 | 293 | MPD, pH 5.6, cubic lipid phase, temperature 293K |
