1XHO
Chorismate mutase from Clostridium thermocellum Cth-682
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 31 |
Unit cell lengths | 48.201, 48.201, 123.693 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 9.960 - 2.200 |
R-factor | 0.198 |
Rwork | 0.194 |
R-free | 0.25500 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.103 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE (2.03) |
Refinement software | REFMAC (refmac_5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.057 | 0.167 |
Number of reflections | 15073 | |
Completeness [%] | 93.3 | 42.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.6 | 291 | 2.5M ammonium sulfate, 0.1M HEPES, pH 7.6, vapor diffusion, temperature 291K, pH 7.60 |