1XHB
The Crystal Structure of UDP-GalNAc: polypeptide alpha-N-acetylgalactosaminyltransferase-T1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-10-09 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 |
| Unit cell lengths | 65.605, 65.605, 125.947 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.390 - 2.500 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.25500 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.500 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.400 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.139 | 0.637 |
| Number of reflections | 18334 | |
| <I/σ(I)> | 2 | |
| Completeness [%] | 97.3 | |
| Redundancy | 3.7 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | PEG 8000, MES, calcium acetate, manganese chloride, AEBSF, EDTA, UDP-GalNAc, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
