1XGF
Backbone Structure of COCOSIN, an 11S storage protein from cocos nucifera
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 2002-01-10 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | H 3 |
Unit cell lengths | 93.659, 93.659, 216.450 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 2.800 - 2.610 |
R-factor | 0.2401 |
Rwork | 0.240 |
R-free | 0.29440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1od5 |
Data reduction software | AUTOMAR |
Phasing software | AMoRE |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.800 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 21429 | |
Completeness [%] | 99.8 | 93.78 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | 20% MPD, Na/K Phosphate buffer containing 7% NaCl, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |