1XG2
Crystal structure of the complex between pectin methylesterase and its inhibitor protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.99 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 90.379, 90.379, 149.095 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 1.900 |
| R-factor | 0.19941 |
| Rwork | 0.198 |
| R-free | 0.23176 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.211 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.082 | 0.338 |
| Number of reflections | 56180 | |
| Completeness [%] | 97.5 | 92.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 294 | magnesium sulfate, MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
