1XFZ
Crystal structure of anthrax edema factor (EF) in complex with calmodulin in the presence of 1 millimolar exogenously added calcium chloride
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 190 |
Detector technology | CCD |
Collection date | 2003-11-28 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 183.258, 316.475, 140.801 |
Unit cell angles | 90.00, 89.91, 90.00 |
Refinement procedure
Resolution | 29.990 - 3.250 |
R-factor | 0.258 |
Rwork | 0.258 |
R-free | 0.27000 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.010 |
RMSD bond angle | 1.700 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.370 |
High resolution limit [Å] | 3.250 | 3.250 |
Rmerge | 0.076 | 0.474 |
Number of reflections | 128284 | |
<I/σ(I)> | 17.3 | 2.2 |
Completeness [%] | 99.0 | 100 |
Redundancy | 4.4 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 278 | PEG400, magnesium chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 278K |