1XFJ

Crystal structure of protein CC_0490 from Caulobacter crescentus, Pfam DUF152

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	2004-08-14
Detector	BRANDEIS - B4
Wavelength(s)	0.9792, 0.9797, 0.94
Spacegroup name	P 1 21 1
Unit cell lengths	39.807, 66.828, 43.170
Unit cell angles	90.00, 110.60, 90.00

Refinement procedure

Resolution	50.000 - 1.750
Rwork	0.180
R-free	0.22000
Structure solution method	MAD
RMSD bond length	0.004
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.810
High resolution limit [Å]	1.750	1.750
Rmerge	0.052	0.125
Number of reflections	21008
<I/σ(I)>	15.8
Completeness [%]	97.8	81.4
Redundancy	7.1	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	PEG 4000, Sodium-acetate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K